I made thin films of polymers and would like to calculate the crystallinity of the polymers using XRD. When I tried to energy minimization my system, I got fatal error as below. I am writing to request some information for making polymer color flakes. I would appreciate the help. I am making polymer composite by solvent mixing method.
When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time Can anyone tell me what the difference between polyethylene oxide and polyethylene glycol is. This might because the pore size of the network in these concentrated polymer solutions was too small to allow the RNA fragments get through. Sample and capillary electrophorrtic conditions were the same as those in Fig 1. Three regimes were observed. Because the radius of gyration of PEO polymer was estimated at about 40 nm, the point of the changing the model was justified.
Three regimes were found from the plot and those were corresponding to Ogstion, reptation, reptation with orientation. The plot was prepared based on the electrophoresis in Fig 1. The plot was prepared based on the electrophoresis in Fig 2. The separation performance of RNA was also evaluated by the resolution length Fig 5. Resolution length tells the minimum, which is smallest RNA nucleotides length resolvable with baseline separation.
Theoretically, two adjacent RNA fragments with 1 nt difference in size could be resolved if the resolution length is equal to 1. It showed that the resolution length varied from Fig 5A It was greatly improved than the resolution length Because the minimum number N min was the nucleotide when the resolution was 1, the resolution 0.
Furthermore, we can find from Table 1 and Table 2 that the threshold in the migration regime moves to the short RNA fragments when the concentration of the polymer increases. The polymer concentration affected the migration time, resolution between RNA fragments. In addition, the minimum resolution length for RNA in the optimal electrophoretic conditions, including the sieving polymer molecular weight, concentration, electric field strength and capillary length, was about 6.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Center for Biotechnology Information , U. PLoS One. Published online May 1. Vipul Bansal, Academic Editor. Author information Article notes Copyright and License information Disclaimer.
Competing Interests: The authors have declared that no competing interests exist. Received Oct 29; Accepted Feb This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. This article has been corrected. See PLoS One. This article has been cited by other articles in PMC.
Abstract The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. Introduction Capillary electrophoresis CE is a powerful technology for nucleic acids separation. Capillary Electrophoresis The CE system had been described in previous works.
Open in a separate window. Fig 1. Capillary electrophoresis of 14 RNA fragments sized from to 10, nt in PEG solution kk with concentration varied from 0. My watch list My saved searches My saved topics My newsletter Register free of charge. Keep logged in. Cookies deactivated.
To use all functions of this page, please activate cookies in your browser. Login Register. Additional recommended knowledge. Sheftel CRC, Krause and G. Bittner PNAS 87 4 : Corpet, G. Parnaud, M. Delverdier, G. Peiffer and S. Tache Cancer Res 60 12 : Borgens and D. Bohnert Journal of Neuroscience Research 66 6 : Bittner el. Neouroscience Letters : Topics A-Z. Why the limit? I'd love to know. Somebody at some point in the past made the decision for the difference [3] and we are stuck with this duality for all eternity.
It undergoes a keto-enol isomerization to form acetaldehyde. The polymer is actually made from the hydrolysis of vinyl acetate, replacing the acetate side chains with hydroxyl groups.
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